cellular retinol binding protein 1  (Proteintech)

Journal: PLoS ONE

Article Title: Altered White Adipose Tissue Protein Profile in C57BL/6J Mice Displaying Delipidative, Inflammatory, and Browning Characteristics after Bitter Melon Seed Oil Treatment

doi: 10.1371/journal.pone.0072917

Figure Lengend Snippet: Proteins differentially expressed in the WAT of HBM mice compared to HS mice identified by MS 1 .

Article Snippet: The primary antibodies, used at a dilution of 1∶1000 in PBS, were mouse antibodies against Xenopus β-actin (Novus Biologicals, Littleton, CO, USA) or bovine cellular retinol binding protein 1 (CRBP1) (Santa Cruz Biotechnology, Dallas, Texas, USA) or rabbit antibodies against human swiprosin 1 (Sigma-Aldrich, St Louis, MO, USA), human extracellular superoxide dismutase 3 (EC-SOD3), human uncoupling protein 1 (UCP1), human γ-synuclein, or human cathepsin D (all from Abcam, Cambridge, UK), human caveolin 1 (CAV1), horse cytochrome c (both from Santa Cruz), and human light chain 3 (LC3) (from Cell Signaling, Danvers, MA, USA).

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Altered White Adipose Tissue Protein Profile in C57BL/6J Mice Displaying Delipidative, Inflammatory, and Browning Characteristics after Bitter Melon Seed Oil Treatment

doi: 10.1371/journal.pone.0072917

Figure Lengend Snippet: Detection of cellular retinol binding protein 1 (CRBP1), swiprosin 1/Efhd2, cathepsin D, extracellular superoxide dismutase 3 (EC-SOD3), uncoupling protein 1 (UCP1), cytochrome c, caveolin 1 (CAV1), γ-synuclein, and light chain 3 (LC3) in the retroperitoneal fat of mice fed the SBO-based high-fat diet alone (HS) or containing low (LBM), medium (MBM), or high (HBM) percentages of BMSO. β-actin was used as the loading control.

Article Snippet: The primary antibodies, used at a dilution of 1∶1000 in PBS, were mouse antibodies against Xenopus β-actin (Novus Biologicals, Littleton, CO, USA) or bovine cellular retinol binding protein 1 (CRBP1) (Santa Cruz Biotechnology, Dallas, Texas, USA) or rabbit antibodies against human swiprosin 1 (Sigma-Aldrich, St Louis, MO, USA), human extracellular superoxide dismutase 3 (EC-SOD3), human uncoupling protein 1 (UCP1), human γ-synuclein, or human cathepsin D (all from Abcam, Cambridge, UK), human caveolin 1 (CAV1), horse cytochrome c (both from Santa Cruz), and human light chain 3 (LC3) (from Cell Signaling, Danvers, MA, USA).

Techniques: Binding Assay, Control